human interleukin 10 il 10  (Boster Bio)

Journal: Molecular & Cellular Proteomics : MCP

Article Title: An Integrative Proteotranscriptomics Approach Reveals New ADAM9 Substrates and Downstream Pathways

doi: 10.1016/j.mcpro.2026.101538

Figure Lengend Snippet: Identification of IGSF8 and IL10RB as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.

Article Snippet: Antibodies used in this study include rabbit anti-ADAM9 (CST, 4151, 1:1000 dilution), mouse anti-ALCAM (Santa Cruz, 74558, 1:200 dilution), mouse anti–interleukin 10 receptor subunit beta (IL10RB) (R&D Systems, MAB874, 5 μg/ml), rabbit anti–Forkhead Box O3 (FOXO3) (CST, 2497, 1:1000 dilution for Western blotting, and 1:100 dilution for immunocytochemistry [ICC]), rabbit anti–immunoglobulin superfamily member 8 (IGSF8) (Sigma, 011917, 1:500 dilution), horseradish peroxidase (HRP)–conjugated rabbit anti-mouse (CST, 7076, 1:2000 dilution), HRP-conjugated goat anti-rabbit (CST, 7074; 1:2000 dilution), HRP-conjugated mouse anti-β-actin antibody (CST, 12262, 1:2000 dilution), and Alexa Fluor 594–conjugated goat anti-rabbit antibody (Thermo Fisher, A-11012, 1:1000 dilution for ICC).

Techniques: Transfection, Expressing, Construct, Western Blot, Two Tailed Test

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: METTL3-mediated m6A modification of CXCR4 drives M2 macrophage polarization and suppresses anti-tumor immunity in esophageal cancer

doi: 10.3164/jcbn.25-180

Figure Lengend Snippet: CXCR4 is highly expressed in TAMs. (A) Flow cytometry detecting the protein expression of CD163 and CD206 on the surface of macrophages. (B) ELISA quantifying IL-10 and TGF-β in co-culture supernatants. (C) qPCR detecting the mRNA level of CXCR4 in macrophages. (D) WB detecting CXCR4 protein in macrophages. (E) MeRIP-PCR detecting the m6A modification of CXCR4. HET-1A: macrophages co-cultured with human normal esophageal epithelial cells; KYSE-450: macrophages co-cultured with human EC cells. * p ≤0.05.

Article Snippet: ELISA kits for IL-10 (E-EL-H6154; Elabscience, Wuhan, China) and TGF-β (JL20082; JONLNBIO, Shanghai, China) were applied to measure the levels of these cytokines in the co-culture system.

Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Modification, Cell Culture

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: METTL3-mediated m6A modification of CXCR4 drives M2 macrophage polarization and suppresses anti-tumor immunity in esophageal cancer

doi: 10.3164/jcbn.25-180

Figure Lengend Snippet: METTL3-CXCR4 axis regulates M2 macrophage polarization. (A) Flow cytometric quantification of CD163 and CD206 on the surface of macrophages. (B) ELISA measurement of IL-10 and TGF-β secretion. * p ≤0.05.

Article Snippet: ELISA kits for IL-10 (E-EL-H6154; Elabscience, Wuhan, China) and TGF-β (JL20082; JONLNBIO, Shanghai, China) were applied to measure the levels of these cytokines in the co-culture system.

Techniques: Enzyme-linked Immunosorbent Assay